ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (4): 716-722.doi: 10.11843/j.issn.0366-6964.2016.04.011

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Establishment and Analysis of ERIC-PCR DNA Fingerprint of Bacteria in Rabbit Manure

LI Yun-fei,QIN Cui-li*,WANG Zhong-ze,YANG Ruo-lan,LI Yang   

  1. (College of Food & Bioengineering,Henan University of Science & Technology,Luoyang 471023,China)
  • Received:2015-03-27 Online:2016-04-23 Published:2016-04-23

Abstract:

This study was designed to apply ERIC-PCR genetic fingerprinting technology to analyze the microbes in rabbit manure,and establish a rapid analysis and detection method of rabbit manure bacterium changes.The ERIC-PCR DNA fingerprint of bacteria in rabbit manure was established by optimizing PCR reaction conditions.The differences between DNA fingerprints of microbiome and dominant strains isolated from rabbit manure was compared,and then the characteristics of the ERIC fingerprint of bacteria in rabbit manure was analyzed.The ERIC-PCR reaction system for bacteria in rabbit manure was established.The characteristic bands for Bifidobacteria were located at 450 bp,the characteristic bands for E.coli were located at 1 300 and 4 000 bp.Anaerobic fermentation experiment proved that the trends of the bands’ brightness were consistent with the results of the colony count (the correlation coefficient between the relative brightness and lg cfu was 0.967 6).A rapid analysis and detection method of rabbit manure bacterium changes by using ERIC-PCR genetic fingerprinting technology was established.This method could reflect the changes of beneficial bacteria in rabbit manure,thus providing a basis for prevention,diagnosis and treatment of rabbit intestinal diseases.

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